irysprep reagent kit (BioNano Genomics)

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BioNano Genomics irysprep reagent kit
Irysprep Reagent Kit, supplied by BioNano Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irysprep+reagent+kit/irysprep+reagent+kit/pm40437759-752-15-19
Average 90 stars, based on 1 article reviews

irysprep reagent kit - by Bioz Stars, 2026-10

90/100 stars

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High Molecular Weight:

Article Title: Chromosome-scale assembly and annotation of the perennial ryegrass genome
Article Snippet: BioNano library preparation and primary steps of optical mapping was performed at the Queen Mary University (London, United Kingdom). .. For preparing libraries, 300 ng high molecular weight genomic DNA was digested by the nicking endonuclease Nt.BspQI (New England Biolabs), and further processed according to the NLRS (Nicks, Labels, Repairs and Stains) protocol of the IrysPrep Reagent Kit (BioNano Genomics, San Diego, USA). .. Labelled and stained DNA was loaded on the Irys chip and subsequently run on the BioNano Irys instrument (30 cycles/run).

Labeling:

Article Title: Genomic analyses reveal the stepwise domestication and genetic mechanism of curd biogenesis in cauliflower
Article Snippet: .. After labeling of nicks using an IrysPrep Reagent Kit (Bionano Genomics) according to the manufacturer’s instructions, the labeled DNA sample was loaded and imaged using the Bionano Saphyr platform (Bionano Genomics), producing ~98.4 Gb raw data with an average length of 270 kb. .. Canu (v.2.2) and the HERA pipeline (v.1.0) were used for de novo genome assembly by producing contigs and merging repetitive regions with PacBio long reads and Illumina paired-end reads.

Article Title: Pan-genome and Haplotype Map of Cassava Cultivars and Wild Ancestors Provide Insights into its Adaptive Evolution and Domestication.
Article Snippet: 921 BioNano 922 Jo urn al Pr e-p roo f The ultrahigh-molecular-weight (uHMW) DNA was isolated from young leaves of Manihot esculenta 923 genotype W14 and AM560 by the Amplicon Express (Pullman, WA, USA). .. The nicked DNA 924 molecules were labeled and stained according to the instructions of the IrysPrep Reagent Kit 925 (Bionano Genomics, San Diego, CA, USA) for NLRS (nick, label, repair, and stain) method as 926 described in detail in Luo et al. (2017). .. The DNA sample was transferred to the nanochannel array 927 of an IrysChip (Bionano Genomics, San Diego, CA, USA) and was automatically imaged by the Irys 928 system (Bionano Genomics, San Diego, CA, USA).

Article Title: Genomic analyses reveal the stepwise domestication and genetic mechanism of curd biogenesis in cauliflower.
Article Snippet: .. After labeling of nicks using an IrysPrep Reagent Kit (Bionano Genomics) according to the manufacturer’s instructions, the labeled DNA sample was loaded and imaged using the Bionano Saphyr platform (Bionano Genomics), producing ~98.4 Gb raw data with an average length of 270 kb. .. Canu (v.2.2)50 and the HERA pipeline (v.1.0)51 were used for de novo genome assembly by producing contigs and merging repetitive regions with PacBio long reads and Illumina paired-end reads.

Article Title: Optical mapping of the Fusarium oxysporum f. sp. melongenae genome.
Article Snippet: The nicking endonuclease enzyme, Nt.BspQ1 (GCTCTTC; New England BioLabs) was preferred to nick DNA of FOMG at particular sequence motifs. .. These nicked molecules of FOMG were first labeled and then stained following the steps of IrysPrep Reagent Kit (BioNano Genomics) as defined in the protocol of Luo et al [26]. .. Irys platform of BioNano Genomics was used to construct an optical map of the FOMG genome.

Staining:

Article Title: Haplotype phased genome of ‘Fairchild’ mandarin highlights influence of local chromatin state on gene expression
Article Snippet: The high-quality HMW DNA molecules were treated with Nb.BssS1 (New England BioLabs, Ipswich, MA) to generate single-strand breaks with sequence-specific motifs (GCTCTTC). .. The nicked DNA molecules were then stained using the IrysPrep Reagent Kit (Bionano Genomics, San Diego, CA) and loaded onto the nanochannel array of IrysChip (Bionano Genomics) following the manufacturer’s guidelines. .. Imaging and data analysis were performed on the Irys system (Bionano Genomics).

other:

Article Title: Symmetric subgenomes and balanced homoeolog expression stabilize the establishment of allopolyploidy in cyprinid fish
Article Snippet: After quality control of mega base-sized DNA molecules, sequence specific labeling of DNA molecules was performed according to IrysPrep Reagent Kit protocol (BioNano Genomics): sequence specificity was provided by Nickase Nt.BspQ1; labeling was carried out by a nick translation process in the presence of a fluorophore labeled nucleotide; labeled nicks were repaired to restore strand integrity; and DNA molecules were stained for backbone visualization.

Article Title: A genome sequence resource for the genus Passiflora, the genome of the wild diploid species Passiflora organensis.
Article Snippet: For BioNano optical genome mapping, ultra-high molecular weight DNA was extracted at the Centre National de Ressources Génomiques Végétales (Toulouse, France), according to the Bionano IrysPrep Reagent Kit protocol (Bionano Genomics).

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